Why Tirzepatide Matters in Peptide Research
Tirzepatide is a 39-amino-acid synthetic peptide with a molecular weight of 4813.5 Da that acts as a dual GIP/GLP-1 receptor agonist. This dual mechanism — engaging both glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1 receptors — is what makes it a focus of preclinical metabolic research rather than a single-pathway GLP-1 analog.
The structural point labs most often overlook is the fatty-acid acylation that extends its half-life. That modification is why sequence purity alone isn’t enough; you also need to confirm the conjugate is intact and the correct molecular mass is present.
Purity Standards That Actually Matter
Research-grade Tirzepatide should carry a third-party HPLC report showing ≥98% purity, backed by mass spectrometry confirming the 4813.5 Da monoisotopic mass. A COA that lists only “HPLC 99%” without a matching MS trace is not independent verification.
- HPLC: purity percentage and impurity profile
- Mass Spectrometry: confirms the 4813.5 Da mass matches the expected sequence
- Amino Acid Analysis: verifies the 39-residue sequence is correct
- Residual Solvent / Endotoxin: for labs that will reconstitute and use in assays
Sourcing Checklist for Bulk Tirzepatide
When buying Tirzepatide in bulk, request a batch-specific COA before payment and confirm the fill weight per vial — 5mg, 10mg, and custom fills are priced differently. Cost-per-mg of verified pure peptide, not headline vial price, is the metric to track.
FAQ
What molecular weight should Tirzepatide be?
Tirzepatide has a molecular weight of 4813.5 Da (C₂₂₅H₃₄₈N₄₈O₆₈). Your mass spec report should match this within the expected measurement tolerance.
What purity should I accept for research-grade Tirzepatide?
Accept ≥98% HPLC purity as the research-grade standard. Material below 97% suggests an incomplete synthesis or purification step and should raise concern.
How is Tirzepatide stored?
Lyophilized Tirzepatide should be stored at -20°C in a vacuum-sealed vial and protected from light. Once reconstituted, keep it refrigerated and use within the supplier’s stated window.
Important: All products are sold strictly for laboratory and research purposes only — not for human or veterinary use. No medical or dosing guidance is provided.