How Are Research Peptides Verified?
Research peptides are verified through three complementary tests — HPLC for purity, mass spectrometry for identity, and amino acid analysis for sequence confirmation. No single test is sufficient on its own, which is why serious suppliers report all three.
HPLC: Measuring Purity
High-performance liquid chromatography separates the peptide from impurities and reports purity as a peak-area percentage, typically requiring ≥98%. It tells you how clean the sample is, but not whether it is the correct peptide.
For a step-by-step guide to reading these reports, see our HPLC report guide.
Mass Spectrometry: Confirming Identity
Mass spectrometry measures the exact molecular weight, confirming the material is actually the intended peptide — a check HPLC cannot provide. This is the test that catches a 98%-pure sample that is nonetheless the wrong molecule.
Amino Acid Analysis: Confirming Sequence
Amino acid analysis breaks the peptide down to verify its amino acid composition, catching sequencing errors that even MS can miss in short peptides. For short sequences like MOTS-c, this extra layer is particularly valuable.
For the full purity framework, see our purity guide. For testing documentation on any batch, contact us on WhatsApp: +852 4419 8097.
Frequently Asked Questions
Q1: What is the difference between HPLC and MS?
HPLC measures purity (how clean the sample is), while mass spectrometry confirms identity (that it is the correct molecule) — they answer different questions and both are required.
Q2: Why is amino acid analysis used?
Amino acid analysis verifies the peptide’s composition to catch sequencing errors that can be missed by HPLC and, in short peptides, by MS.
Q3: What three tests should a COA include?
A complete COA reports HPLC purity (≥98%), an MS spectrum matching the molecular weight, and amino acid analysis confirming the sequence.
Important: All products are sold strictly for laboratory and research purposes only — not for human or veterinary use. No medical, therapeutic, or dosing guidance is provided or implied. Researchers are responsible for compliance with their institution and local regulations.